Explain How Internal And External Business Essay

Explain How Internal And External Business Essay Business is an organization or company that provides goods and services to the community. The general aim of a business is to satisfy the needs and wants of the community whilst also making a profit. In Australia business has a major impact on our everyday lives. Business provides people with employment, it boosts our economy and also it allows us to have a greater quality of life. This means it provides us with the benefits needed to lead a simple and convenient life. There are several factors that can influence a business, these factors include Internal and External influences. Internal influences include factors the business itself may have some control over, whilst on the other hand factors that the business can not control such as laws and regulations are External influences. Importance of business Business is like the blood that flows through our bodies, with out business our lives would be almost impossible. Business plays many different roles in our everyday lives. Most people either own or work for a particular business. Businesses make it possible for the community and even further places such as overseas countries to receive and sell goods and services. A business can be anything that provides goods and services to the community such as corner shops providing customers with goods and sweets and they can also be larger companies such as Nike selling shoes and clothing all over the world. Businesses allow for our economy to grow and become stronger, with out a strong economy a country can not run as smoothly as preferred. One of the main reasons why businesses are so important in our everyday life is due to the fact that businesses provide millions and millions of people with job opportunities. If businesses did not exist people will not be able to work and earn money for e veryday living. There are certain factors that can influence a business. These factors include internal and external influences. Internal influence Internal influences are influences that a business has some control over, such influences include product, location, management, resource management and business culture. 2 Product influences: These influences affect a large majority of the internal structures and operations of a business. Depending on the types of goods and services the business produces will depend on the internal structure. If the goods being produced require certain equipment, these needs must be catered to so that the business may go on with its production. Each type of business has a different set of product influence, for example a service provider will require less preparation, whilst on the other hand a manufacturer will need more services to produce the goods sold. The size of a business can be determined by the different types of goods and services produced and also the availability of technology. So from this we understand that the amount of goods and services produced by a business depends on the size of the business. Poor productivity can be seen in the article Anger at Vodafone Sydney morning herald, this impacts on the business because Vodafone is providing very poor services to their customers, therefore tempting the customers to switch to another network, taking business away from Vodafone. 3 Location influences: One of the most important factors of a business is the location in which that business is positioned in. The location can either make or break the business. Location is especially important for retail and service oriented businesses, as they need to be seen and recognized by customers. The business owner must take into consideration of where he/she chooses to locate their business The business must be in area with a good image, for eg not a poor suburb, they business must be located in a place were customers are consistently walking past and spotting the shop, for eg in the city. Another important thing is that the business must be convenient towards the customers, so that they may take the time and effort to check the place out, if the business is located in a area which is not convenient the customers may loose interest and go somewhere else. 4 Resource influences: -Basically there are 4 main types of resources available to a business, these include: Human resources Information Resources Physical resources Financial resources Human resources may be considered as the most important resource, for they are the employees that allow for the business to run. Information resources are all the actions in which the business takes to research and learn more about their situation for eg sales reports. Physical resources include all the equipment and tools necessary for running the business. Financial resources are basically what the business depends on to buy and help support their business, for example to buy new machines. 5 Management influences: There is no doubt that over time businesses and the way in which businesses are run have dramatically changed. Management has been a part of business that has been changed in an effective way. In the past there were many levels of management and hierarchy in a business that new ideas and issues had to go through, but now due to many factors such as the pressure of competitiveness from globalisation the way in which businesses are run have been altered. These days there are much fewer levels that new ideas and issues have to go through, this will result in quicker and more efficient decisions that in the long run will allow the business to focus on the way it runs. This allows businesses to adapt quicker and more efficiently to the ever changing needs and wants of consumers because there are fewer managers in which the process of decision making must go through. 6 Business culture: In reality every business has its own type of business culture, these are all the values, beliefs and ideas that are agreed upon by all employees and managers of the business. The culture of a business is shown in the way in which the business acts and performs. Business culture is usually shown in the rules and regulations of a business such as the way staff are expected to dress and behave. Culture has been proven to be a necessary tool that must be used by businesses to achieve the best out of its staff members and customers. Business culture is proven to be important in the video Australias food fight: west farmers vs. Woolworths. Coles have changed their business culture by undergoing management change, which has allowed for a different culture to be applied, and this has proven to be successful as they are now basically ahead of Woolworths. 7 Internal influences have an extraordinary impact on business in Australia. They include every thing from were the business is located to how the business is run. The location of a business basically is essential for the success of the business, depending on were it is located, it must be convenient and visible to customers. If it is not located in a convenient area customers may not be able to get to the business and purchase from it. The management of a business is the way in which the business is run, in order to have a successful business it must be under good management and the necessary actions must be taken when they are called for, for eg if employees are needed to be cut off, due to lack of financial sustainability. So basically it is essential for businesses to be affected by internal influences in a positive way. External influences External influences are factors that a business may have little or no control over, such factors include: Economic, financial, geographical, social, legal, political, institutional, technological, competitive situation and markets influences. 8 Economical influences In Australia and the rest of the world, the economy experiences economic cycles. These cycles include booms and busts (recession). Basically during an economic boom the economy experiences higher levels of employment, they may be so confident in their sales that they may increase the price of items to gain higher profits. Also during booms wages of employees tend to increase as more profit is being made by the business. However the economic cycle does have its down side, such as when the economy is experiencing a recession period. An unfortunate reality of recessions is that employment rates decrease due to the fact that the business is not making enough sale profits to keep their employees. Another effect of recession is that business may not be able to raise the price of their products because people are not purchasing so they may have to even lower prices to continue to compete in the market. The influences of economical factors are shown in the article Petrol prices soar and no r elief in site. Due to the poor economical status prices are raised and customers may not be able to purchase petrol at this price. Another ecomical influence can be seen in the radio article impact of floods on grocery prices 2GB, Sydney, this impacts on the business because all the crops are being destroyed making it impossible to make a profit, with farmers not making profits the economy will suffer and be under pressure. 9 Financial influences Financial influences impact a business in several ways. due to deregulation which is basically the extermination of government regulation in an business, Businesses are now able to improve competitiveness with each other. Even better, now due to globalisation, Australia is now able to trade goods overseas, further more improving the financial sustainability of the country. 10 Geographical influences Geographical influences impact businesses in many ways. Australia is located in the Asia-pacific region, the economical statuss in these surrounding countries are very important in the interest of Australia. Basically if china is doing well, it may be able to trade goods and services with Australia creating an advantage for both countries. Another thing is that a lot of Australian companies are located in the Asia-Pacific region, so the countries must be economically doing well for the Australian companies to survive. Also the demography of the country has a significant influence on businesses, for eg Australia must be run to suit people of all different cultures, ages and sexes. One major demographic impact on Australia is the ageing population, with an ageing population there must be centres and facilities created for elders. Also with people retiring, their jobs will need to be completed by younger people, who may not be as experienced as the retired worker. Globalisation is also an important fact of geographical issues, due to globalisation Australia is not tied down just to trade goods and services throughout Australia, but how ever to do so around the entire world. The impact of globalisation can be seen in the video picking up an Aussie apple, we see that Australia is beginning to import overseas apples to Australia. 11 Social influences Social influences have an sever impact on the success of a business. For eg as time goes on peoples taste in fashion and trends will change. If businesses do not adapt to these changes they may be affected in a very negative way. In order for a business to make a profit they must take into consideration all the new fashions and trends going around. One other major social influence is the influence of of our environment which is basically wearing away day by day, businesses must always consider what is in the best interest of the environment, for eg not giving out plastic bags after a sale. One final issue is the issue of family-work practices, as women give birth they must leave work, and this may result in the business losing an employee, affecting in the production of the business. An example of social influences can be seen in the article Petrol price soars and no relief in site, With the rise of petrol, the people in society may decide that instead of using cars, they can probabl y save money by using public transport or riding a bike to work. 12 Legal influences Businesses can not just do as they please. There are many legal actions that influence businesses and basically tell them what they can or cant do. With more and more laws being passed onto businesses, the business must adapt their company to what the law is telling them to do. Generally the people of a society expect that businesses may run under the rules and guidelines provided by the law. Businesses must be aware of all the laws so that they may not breach them and pay the penalties. 13 Political influences Even though political influences may not be the most affective factors onto a business, they still have a pretty heavy impact. For eg whenever a new election Is held, the new government may introduce new policies. A policy that was introduced in 2000 was the goods and services tax, this was a 10 percent tax on the supply of most goods and services used in Australia, this affected businesses in a way that they had to collect taxes basically on behalf of the government. However deregulation has also been introduced in the picture in the past few decades, this is the removal of government regulation in industry, and this takes pressure off businesses and allows for a greater range of competitiveness. 14 Institutional influences Three institutional influences on business are government, regulatory bodies and trade unions and employer associations. Government: Three levels which are federal, state and local. Federal government deals mainly with issues such as tax, making sure that the taxes are being paid and also things such as how the businesses are running in according to the customs regulations. State governments deal with lower case issues such as workers rights and responsibilities such as OHS requirements and making sure businesses are abiding by the state laws and trade practices Local government deals with issues such the condition of building and making sure of improvements, parking rules and fire regulations Regulatory bodies; These bodies observe and understand the way that businesses deal with certain issues and how the business treats its community and consumers. Examples of regulatory bodies include: the Office of fair trading and the Australian competition and consumer commission. (ACCC) Other institutional influences are Trade unions and Australian stock exchanges, these groups have aims such as improving working conditions and pay rates. 15 Technological influences Technology without a doubt has had probably one of the most affects on businesses. With new technologies being invented and discovered every day this can only help improve productivity and efficiency for businesses. Technology has allowed for robotic machinery to be used in productivity which in the long run cheapens the cost of productivity as no employee or has to be paid. Robots also help to reduce the amount of boring and repetitive jobs that some humans may have to do. Also communication technology has been improving rapidly over the past few years making it even easier to communicate with suppliers and consumers, with out them actually being there. Businesses must strive to use the available technology to the best of their ability so that they may keep up or even be ahead of competitors. 16 Competitive situation influences These influences may be very helpful and useful to both consumers and producers, for eg when two businesses are competing to be the market leader, they will lower costs of their stock to attract customers. As a result they will be making more sales and more importantly a profit. These competitive situations also benefit consumers because with competition in a market there will be a greater range of goods available for consumption, this allows the consumer to get a wider variety of choice when considering the good or service they want. Competitive situations are shown in the video Australias food fight: west farmers VS Woolworths as these companies are competing with each other to get the most customer satisfaction, It also discuss how the affects of globalisation such as Aldi here in Australia also competing amongst the big guns. Another example of competitive situation is shown in the article Anger at Vodafone, with Vodafone giving such poor services, customers will want to change to another network providing greater coverage. 17 External influences have a major impact on our every day lives in Australia, with all the different types of factors such as social and technological influences our lives are affected on a day to day basis. If external influences did not exist we would not be living in a society were goods and services from all around the world are accessible to us for our own usage. External influences can be both positive and /or negative for example, competitiveness in the market allows for a variety of options for consumers to choose from but contrasting the effects of external factors such as geographical issues involving the weather can have a negative affect, for eg flooding in farms or drought seasons. Conclusion As mentioned in the executive summary above, business can be compared to the blood in a living organism, it is essential for our everyday survival and living. Business allows us to live the pleasures and entertaining lives that we live each day. Internal influences summed up can be recognized as influences that a business can control whilst external influences are those factors that the business can not control. Internal influences for eg can be explained as factors that a business can use and apply to help determine the success of their business, such as the location in which they choose to place their business. External can be explained by using the example of a economic recession, this can affect the business in many negative ways such as letting employers go. So summed up we can say that business is essential for everyday living and it can be broken down into two different types, Internal and external, and it can be concluded that they basically determine how the business is run and how successful it will be. The end  Ã…’

Gene Therapy Methods and Delivery Systems

Gene Therapy Methods and Delivery Systems Genes the units of heredity â€Å"There can be little doubt that the idea of ‘the gene’ has been the central organizing theme of twentieth century biology† philosopher and biochemist Lenny Moss claimed in 2003. A gene is the basic unit of heredity in a living organism. Genes hold the information to build and maintain their cells and pass genetic traits to offspring. In general terms, a gene is a segment of nucleic acid that, taken as a whole, specifies a trait. The biological entity responsible for defining traits was termed a gene, but the biological basis for inheritance remained unknown until DNA (Deoxyribonucleic acid) was identified as the genetic material in the 1940s. In cells, a gene is a portion of DNA that contains both coding sequences that determine what the gene does, and non-coding sequences that determine when the gene is active (expressed). When a gene is active, the coding and non-coding sequences are copied in a process called transcription, producing an RNA (Ribonucleic ac id) copy of the genes information. RNA can then direct synthesis of proteins via the genetic code. In other cases, the RNA is used directly, for example, as part of the ribosome. The molecules resulting from gene expression, whether RNA or protein, are known as gene products, and are responsible for the development and functioning of all living things. Every cell requires a host of genes that act as blueprints of all the proteins essential for its proper functioning. DNA is a linear polymer of deoxynucleotide monomers. Chemically speaking, it has a double helical structure of two polynucleotide chains held together by hydrogen bonds between the complementary base pairs of the nucleotide strands. Each nucleotide in DNA is comprised of three components, a heterocyclic base, a sugar (2-deoxyribose) and a phosphate group. The nucleotides in a polynucleotide chain are connected through phosphodiester bonds. The nitrogenous bases are of two types, namely, purine based adenine (A) guanine (G), and pyrimidine based cytosine (C) thymine (T). In DNA, base pairs form only between A T and G C and thus the base sequence of each single strand can be deduced from that of its complementary strand. Gene Therapy: Molecular Bandage? Gene therapy is believed by many to be the therapy of the twenty first century because it aims to eradicate cause rather than symptoms of diseases by delivering a normal functioning copy of the mutated gene and its associated regulatory elements into the cell nucleus (1-3). It is a technique whereby an absent or a faulty gene is replaced by a working gene, so that the body can make the correct enzyme or protein and consequently eliminate the root cause of the disease. A potential approach for treating genetic disorders is gene therapy. The most likely candidates for future gene therapy trials will be single gene disorders like, cystic fibrosis, hemophilia, familial hypercholesterolemia, ADA deficiency, Gaucher disease, alpha-1-antitrypsin deficiency etc. Apart from these monogenic disorders, gene therapy also holds the potential of treating acquired diseases such as cancer, by inhibiting oncogene expression or by restoring tumor suppressor genes or through immunomodulation (i.e. by i ncreasing immune response to tumor antigens). Cardiovascular diseases too remain as one of the most â€Å"promising† targets for gene therapy because of the ready accessibility of the vascular system for gene transfer (4). Types of Gene Therapy: Depending on the type of cells into which genes are transferred a process popularly known as â€Å"transfection†. Gene therapy can be broadly classified into two types: Somatic cell Germline gene therapy. Somatic Cell gene therapy: This type of therapy involves the transfection of somatic (non-reproductive) cells especially of those tissues in which expression of the concerned gene is critical for health. Expression of the introduced gene relieves/eliminates symptoms of the disorder, but this effect is not heritable. Germline gene therapy: This type of therapy involves gene transfer into reproductive cells (egg or sperm cells). Here germ cells are modified by the introduction of functional genes, which are ordinarily integrated into their genomes. It would change the genetic pool of the entire human species, and future generations would have to live with that  change. Depending upon the method of correcting the faulty gene, gene therapy can be classified into the following categories: Gene augmentation therapy: This type of therapy is the most appropriate one for the treatment of inherited diseases caused by the loss of a functional gene. It involves supplementing the body cells with a functional copy of the lost gene so that the missing protein is expressed at sufficient levels in the body. It is only suitable if the pathogenic effects of the disease are reversible. Gene inhibition therapy: Its aim is to introduce a gene whose product inhibits the expression of the pathogenic gene or interferes with the activity of its product (5). Suicide gene therapy: This method is best suited for a disease like cancer where the aim is to eliminate a certain population of cells. It involves the transfection of such cells with a suicide gene, whose product is toxic. The suicide genes should be appropriately targeted to avoid widespread cell death (6). Depending upon the mode of delivering genes into a patient’s body, gene therapy can be classified into the following two types: Ex vivo gene therapy: In ex vivo gene therapy, gene transfer occurs outside the patient’s body. This is again sub-divided into two types viz, Autologous Non-autologous. Autologous gene therapy involves the transfection of cells derived from the patient followed by the re-introduction of these cells into the patient’s body. Non-autologous gene therapy involves the transfection of cells not derived from the patient’s body. In vivo gene therapy: In this method the therapeutic gene is directly introduced into the body by injection or by inhalation with the help of a suitably designed vector. Gene Delivery Vectors: Key to Success in Gene therapy Gene therapy, as a novel therapeutic modality, holds enormous promise for the treatment of a multitude of human diseases. However, till date it has failed regrettably in spite of more than 1500 clinical trials completed or currently underway around the world. The primary reason for the failure of the clinical success of gene therapy is the lack of efficient gene delivery agents, commonly referred to as transfection vectors. However, since the biological cell surfaces are negatively charged (due to the presence of glycoproteins and glycolipids containing negatively charged sialic acid residues on cell surface), spontaneous entry of polyanionic naked genes (DNA) into body cells is an inefficient process. Hence â€Å"transfer vehicle† or a â€Å"vector† in needed to condense the macromolecular DNA and to help it in crossing the plasma membrane barrier. Again delivery of therapeutic DNA to the desired body tissue is important to overcome adverse affects. In other words, the problems of developing clinically viable gene therapy methods and designing safe efficient gene delivery reagents are inseparable: shortcomings in one is going to adversely affe ct the success of the other. Hence, realization of the full potential of gene therapy will depend, in a major way, on the future development of safe and efficient gene delivery vectors. The Ideal Vector!!! A â€Å"perfect† or an â€Å"ideal† vector would resemble a traditional pharmaceutical and should have the following characteristics: (a) should be capable of efficiently delivering to its target an expression cassette carrying one or more genes of the size suitable for clinical application, (b) must not elicit an immune response, (c) should not induce inflammation and thus be safe for the recipient, (d) can be produced in bulk at an acceptable cost with reproducibility, (e) should be stable on storage, and finally, it should express the gene (or genes) it carries for as long as required in a strictly regulated manner. No single vector currently available has all these desired properties and each vector presently in use has its own pros and cons. However, it is important to realize that there cannot be a â€Å"universal† vector, optimally useful for all gene therapy applications. This is due to the fact that each disease will have a unique set of technical requirements, and the â€Å"perfect† vector for a specific disease should be optimized in accordance with these requirements. For example, some diseases will require local delivery of the transgene (e.g., ischemia, retinitis pigmentosa, parkinson’s disease, etc.) while others likecancer and atherosclerosis necessitate systemic delivery. In some cases, only a transient, short-lived gene expression will be needed (e.g., therapeutic angiogenesis, cancer) while in monogenic disorders, such as familial hypercholesterolemia, hemophilia and SCID a long term (sometimes life long) gene expression is mandatory (1). The future clinical success of gene therapy will certainly depend on the uphill task of designing â€Å"tailor-made† vector systems for the treatment of specific diseases. The efforts to design a â€Å"perfect vehicle† for the membrane-impermeable DNA have so far led to the development of many methods based on the principles of biology (viral vectors), physics (microinjection, electroporation, particle bombardment, hydrostatic pressure, and ultrasound) and chemistry (synthetic vectors like cationic lipids polymers). Each of these methods has its intrinsic advantages and disadvantages. Viral Vectors: Nature’s Own Infecting Vehicles Viruses have evolved specific mechanisms through the course of evolution to deliver their genetic material into host cells and then hijack the cell’s biosynthetic machinery to produce new viral particles (7). Thus, owing to their natural ability to infect cells, they can be used as vectors in gene therapy by replacing the genes that are essential for replication phase of their life cycle with the therapeutic genes of interest. Majority of the clinical trials currently underway around the world are based on the use of mainly five categories of viruses, namely, retrovirus, adenovirus, adeno-associated virus, lentivirus and herpes simplex virus. Retroviruses: These are a class of enveloped viruses containing a single stranded RNA molecule (approximately 10 kb). Inside the host cell, the RNA is reverse transcribed into double stranded DNA, which in turn integrates into the host genome and is expressed as viral proteins (8). These are the most promising and widely used viral vectors used for gene therapy applications to date. Advantages: Rapidly dividing cancer cells can be targeted by using these viruses. Enters into cells efficiently and gives long lasting gene expression due to stable integration. Disadvantages: Only infects dividing cells, capable of producing tumorigenic mutagenesis due to random integration, unable to deliver larger genomic sequences. Again, it can insert the genetic material of the virus in any arbitrary position in the genome of the host- it randomly shoves the genetic material into a chromosome. Adenoviruses: These are the second most commonly used viruses for gene delivery. They carry a double stranded linear DNA chromosome of approximately 36 kb. Unlike retroviruses, adenoviruses deliver their genetic payload outside the chromosome and are thus less likely to disrupt the cell’s genome (9). But it is immunogenic and may cause inflammation and tissue damage. Adeno-associated viruses (AAV): They contain a single stranded DNA of approximately 4.7 kb surrounded by a protein coat (10) and can integrate at a specific site in human chromosome 19. AAV does not contain any viral genes and contains only the therapeutic gene and it does not integrate into the genome. It requires co-infection with a â€Å"helper† adenovirus for propagation. The advantage of AAV is that it is a non-pathogenic virus but the size for the exogenous DNA it can deliver is limited due to its smaller genome. The difficulty in large scale production is an additional disadvantage. Envelope protein pseudo typing of viral vectors: The envelope proteins on each of these viruses bind to cell-surface molecules make facile attachment to and entry into a susceptible cell. The potential for off-target cell modification would be limited, and many concerns from the medical community would be alleviated. Although viruses are the most efficient gene transfer vehicles available to date, their widespread clinical success has been impeded by the following major drawbacks: (a) Viruses are notorious for eliciting an immune response which, apart from posing a serious threat to the host, also makes a second dose of the same viral vector ineffective due to the production of high level of antibodies against the viral structural components following its initial administration. In 1999, the death of 18-year old Jesse Gelsinger, undergoing gene therapy for ornithine transcarboxylase deficiency, was believed to be triggered by a severe immune response to the adenoviral vector used. (b) Size restriction on the genetic material that can be encapsulated within the viral particles. (c) Possibility of random integration into host genome leading to the risk of inducing tumorigenic mutations (d) Purification of recombinant vector, verifying the sequence, transfecting the packaging cells, isolating and titering the transgenic virus and finally transducing the target cells are time consuming and labor intensive steps. Collectively, all of these complications associated with the use of viral vectors have prompted researchers around the world to develop artificial non-viral transfection vectors. Non-Viral Methods: Although the gene transfer efficacies of the viral vectors are unmatched till date, the above mentioned serious immunogenic concerns associated with their use have led to the development of non-viral methods for gene therapy. The non-viral vectors offer many advantages over their viral counterparts including significantly lower toxicity and immunogenicity, size independent transfer of nucleic acids, very low frequency of integration, relative ease of large-scale production, simpler quality control and substantially easier pharmaceutical and regulatory requirements. The non-viral transfection methods could be broadly classified into two types: Physical methods and Chemical methods. Physical Methods for Gene Delivery: Physical methods involve the direct introduction of genes into the target cells or tissues thereby avoiding the introduction of any foreign substance like a virus or a synthetic vector. Hence, no serious immunogenic concerns are associated with their application. The required genes are inserted via microinjection, electroporation or particle bombardment (gene gun). Microinjection: In this method, the DNA is directly injected into the nuclei of target cells using a fine glass needle under microscope. Although this method is seductively simple, it is difficult to apply clinically. While this method of gene transfer is nearly 100% efficient, it is laborious and time-consuming, typically allowing only a few hundred cells ( Electroporation: This technique involves the perturbation of the cell membrane by an electric pulse for a few microseconds resulting in the formation of transient pores thereby allowing the exogenous DNA to enter the cell cytoplasm. Although there is no limit on the size of DNA that could be delivered via electroporation, the gene transfer efficiency is low and there is high incidence of cell death (12). Gene Gun: In this method, plasmid DNA is coated onto micron size tungsten or gold micro particles and then propelled into cells using either electrostatic force or gas (Helium) pressure. The high velocity results in some DNA being trapped by a few cells and then it may be expressed at sufficient levels. This technique is fast, simple and safe and has been successfully employed to deliver nucleic acids to cultured cells as well as to cells in vivo especially gene transfer to skin (13) and superficial wounds. Chemical Methods of Gene Delivery: DEAE-Dextran: Diethylaminoethyl-dextran (DEAE-dextran) is a polycationic derivative of the carbohydrate polymer, dextran and was one of the first chemical reagents used for transfer of nucleic acids into mammalian cells (14). Owing to its positive charge, DEAE-dextran forms an electrostatic complex with the polyanionic DNA. This technique of delivering genes into cells is simple, reproducible and cost effective. However, it could prove toxic to the target cells especially when DMSO or glycerol is used as a supplementary chemical shock to increase gene transfer efficiency. Secondly, this method is not generally useful for stable transfection studies that require integration of the transferred DNA into the chromosome. A major disadvantage of this method is its ability to transfect a limited variety of cells, e.g. phagocytic cells. Calcium Phosphate: Calcium phosphate co-precipitation method for DNA delivery was first introduced by Graham and Van Der Eb in 1972 (15). This technique involves mixing of DNA with calcium chloride and then carefully adding this mixture to a phosphate buffered saline solution followed by incubation at room temperature. The finely divided DNA containing precipitate thus formed is taken up by the cells via endocytosis or phagocytosis. The main advantages of the calcium phosphate method are its simplicity, low cost, and its applicability to a wide variety of cell types. Moreover, it could be used for transient as well as stable transfection studies. The main drawbacks of the technique involve its sensitivity to slight changes in buffer salt concentrations, temperature, and pH, as well as its relatively poor transfection efficiency compared to newer transfection methods. Cationic Polymers: A wide range of organic polymers has been used for gene transfection, the most popular being polylysine polyethylenimine (PEI) (16). These have a high cationic charge density that condenses DNA into positively charged particles capable of interacting with anionic cell surfaces and entering cells via endocytosis. PEI also exhibits extensive buffering capacity across a wide range of pH which protects DNA inside the endosome from degradation via endosomal swelling and rupture. Dendrimers represent another class of polymers used for gene delivery. They consist of three-dimensional, bifurcated, branched structures called dendrons. The polyamidoamine (PAMAM) family of dendrimers has been shown to be very useful for transfection (17). Cationic Liposomes: â€Å"The Artificial Fat Bubbles† Liposomes, in general, have long been viewed as bio-compatible drug/gene delivery reagents owing to their structural similarity to cell membranes. They are spherical bilayers composed of individual lipids enclosing a watery interior. Each lipid possesses a hydrophilic head group attached via a linker to a large hydrophobic domain. When exposed to an aqueous environment, these amphiphiles spontaneously form large spherical structures known as liposomes above a certain critical vesicular concentration (CVC). Within the sphere, lipids are arranged back-to-back in bilayers with the polar hydrophilic group facing outwards shielding the hydrophobic domain from the aqueous solution. Liposomes may be unilamellar (composed of a single bilayer) or multilamellar (composed of many concentric bilayers). The multilamellar liposome (MLV) upon sonication followed by repeated extrusion through polycarbonate membranes of defined pore size assume the size of small unilamellar vesicle (SUV, 30-100 nm) o r large unilamellar vesicle (LUV, 150-250 nm) (Figure 1).

Personal Narrative- Girls Basketball Game Preparation Essay -- Persona

Personal Narrative- Girls Basketball Game Preparation Basketball seems to get into your blood. It is said by those who play, "You eat, you sleep and you play basketball." When I was a little girl all I wanted to be was a professional basketball player. I couldn't count how many times I pounded that dumb ball in our driveway until it was too dark to see, then I would play for hours more by the porch light. Now I realize that was only childish insanity. Back then they didn’t even have a girls’ profession basketball league in the states. However, that didn't stop me from walking away empty handed. One thing I learned was how to prepare oneself for life. Well, at least, how to prepare oneself for a game. "Offense, defensive rebound-all five. Fast break into secondary. Plays we can run; Motion, Mexico, and Jordan in man defense. Navy and Red against zone. Out of bounds plays; 88, 8 Stack, Box 1 and 2. Remember to offensive rebound, four and one," I said quietly out loud to remind myself while sitting on the sweaty locker room bench. "Now defense, I need to press, stop fast break, and box out. We can do man hard, man soft, zone 2-3 or 3-2." I went on rambling as I finished getting ready. "I can't believe this is our last home game," whined Mindy. Shannon added, "Yeah, the season flew by so fast this year," while tying her lace up. "Well, at least this isn't your last game. You guys all have another game to play," I complained as usual. "Hey, I see no one is twisting your arm off, making you go to Florida, where it is such a dreadful place, always sunny and warm. What a pity. I feel so sorry for you," my best friend Rachel said teasingly. Just then Coach popped her head in the locker room. "All right girls, you ... ...ft," Coach said as we hurried out of the health room. She was right. There was only half the time left. Warm ups seemed to fly by, and before I knew it there were only 35 seconds left on the clock. Murray's team had already headed for the bench for their coach’s last second instructions. "Let's bring it in girls," yelled Coach Moore from our bench. We all ran over not wanting to miss any important last minute words of wisdom from Coach. "OK, starters check in, you know who you are. As I have said, they are about equal in ability with us. We need to go out there and play our hearts out tonight, girls. Enjoy this, it is your last home game this season," Coach said. With that the Ref. blew his whistle to signal the start of the game. I, with the rest of the starters, walked out onto the court and got ready for the tip off, knowing I was completely prepared for the game. Personal Narrative- Girls Basketball Game Preparation Essay -- Persona Personal Narrative- Girls Basketball Game Preparation Basketball seems to get into your blood. It is said by those who play, "You eat, you sleep and you play basketball." When I was a little girl all I wanted to be was a professional basketball player. I couldn't count how many times I pounded that dumb ball in our driveway until it was too dark to see, then I would play for hours more by the porch light. Now I realize that was only childish insanity. Back then they didn’t even have a girls’ profession basketball league in the states. However, that didn't stop me from walking away empty handed. One thing I learned was how to prepare oneself for life. Well, at least, how to prepare oneself for a game. "Offense, defensive rebound-all five. Fast break into secondary. Plays we can run; Motion, Mexico, and Jordan in man defense. Navy and Red against zone. Out of bounds plays; 88, 8 Stack, Box 1 and 2. Remember to offensive rebound, four and one," I said quietly out loud to remind myself while sitting on the sweaty locker room bench. "Now defense, I need to press, stop fast break, and box out. We can do man hard, man soft, zone 2-3 or 3-2." I went on rambling as I finished getting ready. "I can't believe this is our last home game," whined Mindy. Shannon added, "Yeah, the season flew by so fast this year," while tying her lace up. "Well, at least this isn't your last game. You guys all have another game to play," I complained as usual. "Hey, I see no one is twisting your arm off, making you go to Florida, where it is such a dreadful place, always sunny and warm. What a pity. I feel so sorry for you," my best friend Rachel said teasingly. Just then Coach popped her head in the locker room. "All right girls, you ... ...ft," Coach said as we hurried out of the health room. She was right. There was only half the time left. Warm ups seemed to fly by, and before I knew it there were only 35 seconds left on the clock. Murray's team had already headed for the bench for their coach’s last second instructions. "Let's bring it in girls," yelled Coach Moore from our bench. We all ran over not wanting to miss any important last minute words of wisdom from Coach. "OK, starters check in, you know who you are. As I have said, they are about equal in ability with us. We need to go out there and play our hearts out tonight, girls. Enjoy this, it is your last home game this season," Coach said. With that the Ref. blew his whistle to signal the start of the game. I, with the rest of the starters, walked out onto the court and got ready for the tip off, knowing I was completely prepared for the game.

Abortion is not Murder in the right circumstances Essay -- essays rese

Abortion Is Not Murder under the Right Circumstances She was only twelve, only for a walk on the street right by her house when he got to her. The experience was terrifying and all she wanted to do was forget about it, forget about him on top of her, making her do things that should only be done with someone she loves. The rape is finally over and he lets her go after making her promise not to tell. A few weeks after the rape she still hadn’t told, but started feeling woozy and nauseous in the morning; she went and consulted in her mother. After waiting out what she believes was a stomach flue for another few weeks she saw that her belly started to grow. She finally told her mother what had happen and the little twelve year old girl was 4 weeks pregnant. A heated discussion among students, parents and government officials is whether abortion should be legal. I recently read an article about a twelve year old girl who had been raped and had her child because no one gave her the option of abortion, her family felt that she would e mbarrass the family because it was morally wrong. Would it have been wrong for this child to theoretically murder her newborn? Murder is the act of taking another’s life with malicious thoughts; it is also taking the life of another human being without medical supervision and by brutal ways. I believe that abortion is not murder if none of those three apply, in the right circumstance, abortion is not murder. Nuss-2 Abortion is the act of terminating...

How to Read Literature Like a Professor Chapter Analysis Essay

Chapter 1: Every Trip Is a Quest (Except When It’s Not) In Chapter 1 the author explains the symbolic reasoning of why a character takes a trip. They don’t just take a trip they take a quest. Structurally a quest has a quester, a place to go, a stated reason to go there, challenges and trials en route, and a reason to go there. Quests usually involve characters such as a knight, a dangerous road, a Holy Grail, a dragon, an evil knight, and a princess. The quest also involves the character to gain self-knowledge out of taking the adventure to the stated place where he or she is going. Chapter 2: Nice to Eat with You: Acts of Communion Chapter 2 tells of the symbolism that takes place while characters are eating a meal together. The author states that when people eat together it is saying â€Å"I’m with you, I like you, we form a community together.† The meal also shows how a person feels towards another person. It can show whether you like or dislike the person. The author explains how the description of the food isn’t just to inform you of what is being eaten. It is to draw you into the moment and help you feel the realism of that moment. Chapter 3: Nice to Eat You: Acts of Vampires In Chapter 3 the author explains in Chapter three how vampirism isn’t always about vampires. Vampirism is a characteristic a character can portray, such as selfishness, exploitation, and rudeness. The character takes advantage of people, like a vampire would to his prey. Many authors actually use vampires, ghosts, or doppelgangers to portray vampiristic qualities instead of letting the reader infer those qualities into a human. Chapter 4: If It’s Square, It’s A Sonnet Chapter 4 tells about how sonnets are formed and how to identify a sonnet. Sonnets are in a square shape and they always have 14 lines in them. The author says that sonnets may be challenging to understand, but they are the most interesting poems because they are able to say what they have to say in only 14 lines and 10 syllables. Chapter 5: Now, Where Have I Seen Her Before? In chapter 5 the author explains how stories overlap in a way. Book are never totally original. They all use similar characters with similar personalities. Authors use other authors to influence their style of writing and what they write about. Chapter 6: When In Doubt, It’s from Shakespeare . . . Chapter 6 is all about William Shakespeare. The author believes that almost all stories written were somehow influenced by a play or sonnet or some sort of Shakespeare’s works. Some of even the most famous stories ever written were somehow connected to a piece by William Shakespeare. Chapter 7: . . . Or the Bible Chapter 7 is similar to the chapter that refers to Shakespeare. It states how every piece of literature is somehow related to or referring to the Bible. They all involve things such as temptation, betrayal, denial, etc. Also, writers refer to the Bible because almost everybody knows at least some of the stories from the Bible. Chapter 8: Hanseldee and Greteldum In chapter 8 the author explains how many stories are connected to fairy tales, like a parallel. Fairy tales are easy to connect to because they all have a plot and solution, so there is always a way to connect a story to the fairy tales. Chapter 9: It’s Greek to Me Chapter 9 is about myths and how they are related to Greek mythology. There are three types of myth: Shakespearean, Biblical, and fairy tales. There are many things connected to Greece. Many things are named and based off of Greek characters. Mascots, towns, and some people are even named after some of the greates heros of Greek times. Chapter 10: It’s More Than Just Rain or Snow Chapter 10 is about the symbolism of weather. The author talks of the uses of weather such as rain, wind, snow, etc. The uses are plot device, atmospherics, democratic element, cleansing, and restorative. The weather is a very critical detail in setting the scene for stories. Chapter 11: More Than It’s Gonna Hurt You: Concerning Violence Chapter 11 is about the significant meaning of violence. There are two categories of violence in literature: the specific injury and the narrative violence. Specific injury causes characters to visit on one another or on themselves. Narrative violence cause the characters to cause harm in general. Chapter 12: Is That a Symbol? Chapter 12 is about symbolism. The author says that not everybody will think a symbol will mean the same thing and it won’t. The symbol is whatever you think it means. Some writers make their symbols direct, but most let you use your own imagination. Chapter 13: It’s All Political Chapter 13 was about how most writing is political. It was about how writers secretly put their political point of views into their stories. Usually political writing is boring and vague. Some writing is more political than others, but nearly all writing is political on some level. Chapter 14: Yes, She’s a Christ Figure, Too Chapter 14 is about how almost everything, in some form, is a Christ figure. The chapter gives a list to relate characters to. The list is 1. crucified, wounds in the hands, feet, side, and head 2. in agony 3. self-sacrificing 4. good with children 5.good with loaves, fishes, water, wine 6. thirty-three years of age when last seen 7. employed as a carpenter 8. known to use humble modes of transportation, feet or donkeys preferred 9. believed to have walked on water 10. often portrayed with arms outstretched 11. known to have spent time alone in the wilderness 12. believed to have had a confrontation with the devil, possibly tempted 13. last seen in the company of thieves 14. creator of many aphorisms and parables 15. buried, but arose on the third day 16. had disciples, twelve at first, although not all equally devoted 17. very forgiving 18. came to redeem an unworthy world. Chapter 15: Flights of Fancy Chapter 15 is all about the symbolism of flying. Flying is freedom. That’s what it symbolizes. Usually stories are fiction when you see a character flying, but when you do the person is either a superhero, ski jumper, crazy, a circus act, suspended on wires, an angel, or heavily symbolic. Chapter 16: It’s All About Sex . . . It’s All About Sex, or Chapter 16, is about the symbollic meaning of sex in a story or movie. The author says that usually sex isn’t even about the sex or things that have nothing to do with sex are usually about sex. Chapter 17: . . . Except Sex Chapter 17 is about how sex is never actually used in literature. Authors always describe the scene before and the scene after but never the inbetween. There is really only one way of writing about sex, of course the characters can do different things but in the end it’s still the same thing. that’s why it’s ok to just leave the actual physical part out of the book and only describe the before and after. Chapter 18: If She Comes Up, It’s Baptism Chapter 18 is all about the significance of water. When a character goes under water and comes back up, it usually means that the character was renewed or reborn. Then after that the character will be a whole new being. Sometimes the character goes under and doesn’t come back up, but the only significance this has is that the character dies. Chapter 19: Geography Matters . . . Chapter 19 is about the geography. The geography symbolizes the mood of the characters. Such as if a character is on the beach they are usually relaxed and calm. Hills can symbolize journeys or difficult tasks that the character has to overcome. Chapter 20: . . . So Does Season Chapter 20 is about the effect seasons have. Most teenage movies are set in the summer because that is supposedly the prime of their lives. Winter signifies age and the characters are usually moody or gloomy. When it is fall the characters are probably changing is some ways. Spring the character is probably renewed in some way. Chapter 21: Marked for Greatness Chapter 21 is about how defaults in a character mean more than just some sort of handicap. Everything has a meaning. Take for instance the scar on Harry Potter’s head. It has a story of its own. It reminds him of his parents and what happened to them. Chapter 22: He’s Blind for a Reason, You Know Chapter 22 is about the symbolic meanings of blindness. Not all the time in stories is the person who is literally blind actually the blind one. Blind people can be the people who have sight but are greedy and selfish. Chapter 23: It’s Never Just Heart Disease . . . Chapter 23 tells about how when an author mentions heart disease in a story, it’s never just heart disease. Since the heart represents practically all emotion, when the heart has troubles it could be interpreted as lonliness or pain. Usually the characters with heart disease are never happy with life. Chapter 24: . . . And Rarely Just Illness In chapter 24 the author suggests the use of illnesses. He states that when writing a story, you can’t just use any illness off the top of your mind. The illness must be picturesque, meaning that the illness should affect the physical appearance of the character. Also the illness should have strong symbolic or metaphorical possibilities. Chapter 25: Don’t Read with Your Eyes Chapter 25 is called â€Å"Don’t Read With Your Eyes.† The chapter is basically a restatement of previous chapters, just repeating that readers need to think of the story in a different perspective and that they should see the author or maybe the characters point of view. Chapter 26: Is He Serious? And Other Ironies In chapter 26 the author states that even though we went through the entire book learning about all of these new symbolic meanings in literature, sometimes irony will come and mess things all up. He says something that helps me remember: â€Å"irony trumps everything.† Even though we spent hours reading about all of the symbolic and metaphorical possibilities in stories, there only has to be one, tiny ironic thing that could come along at the end of the story and completely wipe out all of the symbols and metaphor we just read.

What is life

What is emotional state? Is it a secret? Is it an adventure? Is it a way to dish out others? Or is it so brief comp atomic number 18d to the lie in of the measure in the universe it has no message at all? pile do been wondering this for thousands of years, just no peerless has found an answer. M all enumerate for complicated ways to ensure sustenance, yet the answer is so simple life is a book. Since the day the binding was undecided for the first time,and the author began writting the story each thought, action, and emotion that is experienced is written into the book. reminiscence is just re rendition the book.Some pages torn, stuck together, smeared so we cornerstonet remember it as well, further it is all writtten down. Every hope, all dream, every(prenominal) fear is recorded in time forever. Some books have flashy, tacky covers, provided tell the most trouble nigh stories on the inside. Some have dull, nondescript covers, entirely tell the warmest, most ad venturous stories that causes one to recall some earlier chapter in ones own life. Every experience is a chapter. Some chapters utter of friends and family, some let loose of the most horrible fears and the most ideate about opes, and some tell of dear and Loss that the author experienced.Some chapters are not finished and will be completed at a subsequently time. Some chapters are blurred, because not change surface the author knows what happened, or what he wishes would happen. And some chapters are blank pages, waiting to be filled. More than once, the author has wished he could go back up a few pages and fiat it divers(prenominal)ly, merely alas, the book has already been published, and backnot be changed. As the book ages, it loses some of its shine, pages hang-up and fade, and the binding blend ins loose.Some pages suddenly become so clear, you cant understand why you didnt see it before, fleck others become so transparent that you cant fifty-fifty remember readin g them. And after the book has become so old that it cant even be read or travel without falling apart, it is taken out of circulation and stored. non only in a personal place, but in peoples heart, those who loved the book as if it was their dress hat friend. It is stored with every other book of every persons life back before humanity could even speak with more than grunts and body langauge. It is stored in the spacious Library.As you look around this glorous library, the books down higher than any mountain, and strech farther than any eye can see. And on every shelf are books, and in the spirit of the room are thousands of books open to various pages and chapters recording the authors dictations from the slit of the book until it is finished and published. And then in that respect are the old books that are creation called out of circulation, catalogued and stored. You reach out and get down reading one of the many books. It is the life of your friend. You take notice of how many multiplication your own book overlaps with your riends book, and how sympathetic the stories are.As you read this book, you see the meaning of life, not what the stories are, or what the cover looks like, but how every book tells a different story and while many whitethorn overlap and share dreams, hopes, and fears, they each chink knowledge and together touch on up life. You see, you cant sample a book by its cover, nor can you judge a person by appearance, each complete with fears, dreams, joys, sorrows, memories, friends and familes. Together we all make up life, and our stories inspire others to grow and to have the best story they can.

Nobel Prize Winner: James Watson

Among the close non open and controversial Nobel appreciate recipients is throng Watson. He, unitedly with Francis crick and Maurice Wilkins, was awarded the Nobel jimmy in the year 1962 in the Physiology or Medicine category. He is one of the scientists who find the molecular(a) bodily social system of the desoxyribonucleic acid which is hailed as one of the bulky break by dint ofs in the compass of Sciences.James Dewey Watson was born on April 6, 1928 in Chicago, Illinois. In his archaeozoic life, he was state to be fond of bird-gazing in concert with his father. When he was 12 years old, he was set ab go forth of the famous radio show entitle essay Kids, a game which ch aloneenged young students to a prove contest. At the age of 15, Watson entered the University of Chicago with the suffice of the thuslyce liberal policy of Robert Hutchins, the University president. In 1946, his interest in the bowl of science changed from his former concern on ornithology to tra nsmitt fittings after he had read What Is behavior? by Erwin Schrodinger. In 1947, he received his knight bachelors degree in fauna from the akin university.Among Watsons foremost influences was Salvador Luria, as well as a Nobel kale winner. He was engrossed to the last mentioneds turn which exposed him on the temperament of genetical mutations. In the first months of 1948, Watson started to pursue his Ph.D. explore at indium University at Lurias science lab. He was equal to(p) to pick up with an former(a) Nobel calculate recipient Max Delbruck. Delbruck and Luria were the pioneers of the phage Group, a movement of geneticists who underwent studies and researches on microbial genetic science.Eventually, Watson was as well able to cast with the group as a working(a) scientist. His experience with the phage group unfastened up his scientific association on the nature and building of genes. In order to develop his fellowship ab pop step forward the components of a gene, he took a strain with an some other scientist Feliz Haurowitz in 1949 in which he was able to gain the conventional views on genes such(prenominal)(prenominal) as genes were proteins and vice versa that genes have the capacity to replicate themselves and other scientific data about the desoxyribonucleic acid. besides by means of with all those knowledge, Watson was also fascinated with the work of Oswald Avery explaining that deoxyribonucleic acid was thence the genetic molecule. Towards his doctoral studies, he underwent roentgenograms researches which act to de-motivate bacterial viruses. In 1950, he received his Ph.D. in Zoology at indium University.To be able to recruit to a greater extent his knowledge on genetics and genetic composition, Watson went to Europe to pursue a postdoctoral study. He confronted at the laboratory in Copenhagen owned by a biochemist named Herman Kalckar whose studies were also linked with the nucleic acids. through with(predicate) hi s stay with Kalckars laboratory, he was able to conduct experiments with other component of the Phage group, Ole Maaloe.The latters studies and researches were interested on the desoxyribonucleic acid and the earlier supposition that it is the genetic molecule. In a impacting in Italy where he come with Kalckar, he met Maurice Wilkins who was also a inclined geneticist. In an event, Wilkins had shown Watson an roentgenogram diffraction data for desoxyribonucleic acid (which was primarily worked out by Rosalind Franklin). After seeing the X-ray, he came to a conclusion that desoxyribonucleic acid had a evident complex body part. Watson attempt to discover this through his experimental research make in different universities.Watson came to a finality to be familiar with performing X-ray diffraction experiments for the contend that such undertaking would lead him to a more(prenominal) probable and easier discovery of the DNA structure (He was exalt by Linus Pauling who was able to publish the protein alpha lock gravel with his unremitting efforts in allowing X-ray experiments on molecular forge.In 1951, Watson, together with Francis twist and Maurice Wilkins, started to work out a series of experimental researches using Franklins X-ray findings on DNA structure. A disceptation was said to occur du work party the period of experimental researches among Watson, Crick and Wilkins for the intellect that they were using Franklins data and findings without the latters knowledge and consent.To further study Franklins X-ray findings, Watson attended one of Franklins seminar by which she explained how she obtained her findings on the DNA structure. Originally, Franklin claimed that the DNA was structured in volute-form. With this, Watson had started again to construct a molecular imitate that in the end it was criticize by Franklin by saying that the phosphate vertebral columns must not be in the inside but on the outside.Eager to wind their attempt, the twain utilise Franklins observations in their last-ditch attempt to arrive at the DNA structure position. However, 1951, the absolute details of the chemic structure of the backbone of the DNA were identified by Alexander Todd, a biochemist. With that, Watson and Crick were asked to stop working with the DNA structure in 1952. Yet the two had never all in all institutionalise aside their trust to come up with the DNA structure example.After legion(predicate) trips which exposed Watson and Crick to different methods and experimental systems that could help them in their government note on DNA structure model, they were again asked to run working on the DNA morphological model by the then laboratory director Maurice Wilkins. Through the years, Franklins findings progresses and even developed. The two, again, used Franklins findings in their experimental research on the DNA structure.The most outstanding contribution of Watson in the inbuilt out of bounds of the structural model of the DNA was his discovery of the groundwork base pairs. These base pairs argon said to be the chief answer in solving the structure and function of the DNA. Watson used the Pauling tradition, which he was formerly exposed.On February 1953, Watson worked out a molecule model which used a straight periphery, and exacto blade, white artificial and adhesive. He make the molecules flat in their eyehole so that he could slide the cardboard models and chit-chat how they work. Through such improvised models, Watson saw that the bigger two ring (A and G nucleobase also referred as the purines) could be matched with a lesser one ring (T and C nucleobases also referred as the pyrimidines).Watson hypothesized if the tow pairs could be paired through a hydrogen bond certificate which he discovered possible. He then spy that the two pairs could be perplexd over on each other with alike general configuration. To elaborate, the hexagonal rings were of import and the comparative hightail its of the five-member rings of A and G were the identical. Watson perceived that numerous members were falling into place such that he regarded it as the answer. He was amend for formulating such conclusion. Watsons discovery of the base pairs was firm with what Chargaff, also a biochemist, had already worked out.Not so foresightful that Watson and Crick had stainless their experimental research on the structural model of DNA by last(a) the double helix form of the DNA. They presented their findings through a journal entitled Nature. With this great discovery, Watson and his co-scientists Crick and Wilkins were inclined the Nobel valuate in 1962 for their discovery of the structure of nucleic acids.Nevertheless, as mentioned earlier the hostility involving the original works of Franklin had put the three Nobel Prize Awardees in so lots criticisms primarily with their chastisement to acknowledge the contribution of Franklin. But Watson took the courage to crystallise the issue and appraise Franklins involvement in the discovery of the DNA structural model. In 1968, he published a phonograph recording entitled The Double- coil which explained his groups side regarding the joust with Franklin.He clarified that it was not intentional to swing Franklin all throughout their achievements. He said that Franklin was really one of the persons who gave him the impetus to hand harder and make him more careful in analyzing his experimental research on DNA structure. In the end, his aggroup included Franklin as one of the most in-chief(postnominal) persons behind the achiever of their structural model of DNA.Watsons published hold back made the public fix how scientists like him undergo so much hardships for the sake of scientific discoveries which go off really aid the entire mankind in uplifting the quality of their lives. He had proven that with great effort and outstanding sacrifice, anyone could victoriously achieve his or h er goals.Watson did not stop his scientific endeavors with his Nobel Prize award. He worked with the Genome Project in 1988 which he held up until 1992.ReferencesHamilton, J. (2004). James Watson Solving the Mystery of DNA (Nobel Prize-Winning Scientists). Enslow Publishers.Watson, J. D. (2001). The Double Helix A Personal study of the Discovery of the expression of DNA (First ed.). Touchstone.Nobel prize winner james watsonAmong the most notable and controversial Nobel Prize recipients is James Watson. He, together with Francis Crick and Maurice Wilkins, was awarded the Nobel Prize in the year 1962 in the Physiology or Medicine category. He is one of the scientists who discovered the molecular structure of the DNA which is hailed as one of the great breakthroughs in the field of Sciences.James Dewey Watson was born on April 6, 1928 in Chicago, Illinois. In his early on life, he was said to be fond of bird-gazing together with his father. When he was 12 years old, he was lineame nt of the famous radio show entitled test Kids, a game which challenged young students to a try out contest. At the age of 15, Watson entered the University of Chicago with the help of the then liberal policy of Robert Hutchins, the University president. In 1946, his interest in the field of science changed from his former concern on ornithology to genetics after he had read What Is animation? by Erwin Schrodinger. In 1947, he received his unmarried mans degree in Zoology from the comparable university.Among Watsons foremost influences was Salvador Luria, also a Nobel Prize winner. He was engrossed to the latters work which exposed him on the nature of genetic mutations. In the first months of 1948, Watson started to pursue his Ph.D. research at Indiana University at Lurias laboratory. He was able to meet with another Nobel Prize recipient Max Delbruck. Delbruck and Luria were the pioneers of the Phage Group, a movement of geneticists who underwent studies and researches on micr obial genetics. Eventually, Watson was also able to work with the group as a working scientist.His experience with the Phage group undecided up his scientific knowledge on the nature and structure of genes. In order to develop his knowledge about the components of a gene, he took a course with another scientist Feliz Haurowitz in 1949 in which he was able to gain the conventional views on genes such as genes were proteins and vice versa that genes have the capacity to replicate themselves and other scientific data about the DNA. But through with all those knowledge, Watson was also fascinated with the work of Oswald Avery explaining that DNA was indeed the genetic molecule. Towards his doctoral studies, he underwent X-rays researches which attempted to de-motivate bacterial viruses. In 1950, he received his Ph.D. in Zoology at Indiana University.To be able to enhance more his knowledge on genetics and genetic composition, Watson went to Europe to pursue a postdoctoral study. He sta yed at the laboratory in Copenhagen owned by a biochemist named Herman Kalckar whose studies were also linked with the nucleic acids. Through his stay with Kalckars laboratory, he was able to conduct experiments with another member of the Phage group, Ole Maaloe.The latters studies and researches were concerned on the DNA and the earlier supposition that it is the genetic molecule. In a meeting in Italy where he accompanied Kalckar, he met Maurice Wilkins who was also a devoted geneticist. In an event, Wilkins had shown Watson an X-ray diffraction data for DNA (which was originally worked out by Rosalind Franklin). After seeing the X-ray, he came to a conclusion that DNA had a distinct structure. Watson attempted to discover this through his experimental research done in different universities.Watson came to a decision to be familiar with performing X-ray diffraction experiments for the reason that such undertaking would lead him to a more probable and easier discovery of the DNA st ructure (He was inspired by Linus Pauling who was able to publish the protein alpha helix model with his unremitting efforts in undergoing X-ray experiments on molecular model.In 1951, Watson, together with Francis Crick and Maurice Wilkins, started to work out a series of experimental researches using Franklins X-ray findings on DNA structure. A controversy was said to occur during the period of experimental researches among Watson, Crick and Wilkins for the reason that they were using Franklins data and findings without the latters knowledge and consent. To further study Franklins X-ray findings, Watson attended one of Franklins seminar by which she explained how she obtained her findings on the DNA structure.Originally, Franklin claimed that the DNA was structured in helix-form. With this, Watson had started again to construct a molecular model but in the end it was criticize by Franklin by saying that the phosphate backbones must not be in the inside but on the outside. Eager to finish their attempt, the two used Franklins observations in their ultimate attempt to arrive at the DNA structure model. However, 1951, the absolute details of the chemical structure of the backbone of the DNA were identified by Alexander Todd, a biochemist. With that, Watson and Crick were asked to stop working with the DNA structure in 1952. Yet the two had never completely put aside their desire to come up with the DNA structure model.After numerous trips which exposed Watson and Crick to different methods and experimental systems that could help them in their account on DNA structure model, they were again asked to continue working on the DNA structural model by the then laboratory director Maurice Wilkins. Through the years, Franklins findings progresses and even developed. The two, again, used Franklins findings in their experimental research on the DNA structure.The most outstanding contribution of Watson in the entire pursuit of the structural model of the DNA was his disc overy of the nucleotide base pairs. These base pairs are said to be the chief answer in solving the structure and function of the DNA. Watson used the Pauling tradition, which he was formerly exposed.On February 1953, Watson worked out a molecule model which used a straight periphery, and exacto blade, white cardboard and adhesive. He made the molecules flat in their loop so that he could slide the cardboard models and inspect how they work. Through such improvised models, Watson saw that the bigger two ring (A and G nucleobase also referred as the purines) could be matched with a lesser one ring (T and C nucleobases also referred as the pyrimidines).Watson hypothesized if the tow pairs could be paired through a hydrogen bond which he discovered possible. He then observed that the two pairs could be placed over on each other with alike general configuration. To elaborate, the hexagonal rings were central and the comparative courses of the five-member rings of A and G were the identi cal. Watson perceived that numerous members were falling into place such that he regarded it as the answer. He was right for formulating such conclusion. Watsons discovery of the base pairs was unswerving with what Chargaff, also a biochemist, had already worked out.Not so long that Watson and Crick had completed their experimental research on the structural model of DNA by concluding the double helix form of the DNA. They presented their findings through a journal entitled Nature. With this great discovery, Watson and his co-scientists Crick and Wilkins were given the Nobel Prize in 1962 for their discovery of the structure of nucleic acids.Nevertheless, as mentioned earlier the controversy involving the original works of Franklin had put the three Nobel Prize Awardees in so much criticisms primarily with their failure to acknowledge the contribution of Franklin. But Watson took the courage to clarify the issue and appraise Franklins involvement in the discovery of the DNA structur al model. In 1968, he published a book entitled The Double-Helix which explained his teams side regarding the controversy with Franklin. He clarified that it was not intentional to bypass Franklin all throughout their achievements. He said that Franklin was really one of the persons who gave him the impetus to strive harder and make him more careful in analyzing his experimental research on DNA structure. In the end, his team included Franklin as one of the most important persons behind the success of their structural model of DNA.Watsons published book made the public realize how scientists like him undergo so much hardships for the sake of scientific discoveries which can really aid the entire mankind in uplifting the quality of their lives. He had proven that with great effort and outstanding sacrifice, anyone could victoriously achieve his or her goals.Watson did not stop his scientific endeavors with his Nobel Prize award. He worked with the Genome Project in 1988 which he held up until 1992.ReferencesHamilton, J. (2004). James Watson Solving the Mystery of DNA (Nobel Prize-Winning Scientists). Enslow Publishers.Watson, J. D. (2001). The Double Helix A Personal Account of the Discovery of the Structure of DNA (First ed.). Touchstone.